Search results for "nuclear transformation"

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Human Intrinsic Factor Expression for Bioavailable Vitamin B12 Enrichment in Microalgae

2018

Dietary supplements and functional foods are becoming increasingly popular complements to regular diets. A recurring ingredient is the essential cofactor vitamin B12(B12). Microalgae are making their way into the dietary supplement and functional food market but do not produce B12, and their B12 content is very variable. In this study, the suitability of using the human B12-binding protein intrinsic factor (IF) to enrich bioavailable B12 using microalgae was tested. The IF protein was successfully expressed from the nuclear genome of the model microalga Chlamydomonas reinhardtii and the addition of an N-terminal ARS2 signal peptide resulted in efficient IF secretion to the medium. Co-abunda…

0301 basic medicineSignal peptide<i>Chlamydomonas</i>; vitamin B<sub>12</sub>; cobalamin; intrinsic factor; microalgae; nuclear transformation; recombinant protein; dietary supplements; functional foodsChlamydomonaChlamydomonas reinhardtiiArticleGeneral Biochemistry Genetics and Molecular Biologydietary supplements03 medical and health sciencesIngredientnuclear transformationFunctional foodpolycyclic compoundsVitamin B12Food sciencecobalaminlcsh:QH301-705.5functional foodsIntrinsic factorGeneral Immunology and MicrobiologybiologymicroalgaeChlamydomonasChlamydomonasnutritional and metabolic diseasesvitamin B12biology.organism_classificationBioavailability030104 developmental biologylcsh:Biology (General)dietary supplementintrinsic factorGeneral Agricultural and Biological Sciencesrecombinant proteinBiology; Volume 7; Issue 1; Pages: 19
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